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National eDNA-based monitoring of Batrachochytrium dendrobatidis and amphibian species in Norway

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National eDNA-based monitoring of Batrachochytrium dendrobatidis and amphibian species in Norway

by
Osman, Omneya Ahmed; Andersson, Johan; Martin-Sanchez, Pedro M.; Eiler, Alexander

Publication date
Usage
Attribution 4.0 InternationalCreative Commons Licenseby
Topics
environmental monitoring, invasive species, Metabarcoding, qPCR, risk assessment
Publisher
Pensoft Publishers
Collection
biodiversity
Contributor
Pensoft Publishers
Language
English
Rights
https://biodiversitylibrary.org/permissions
Rights-holder
Copyright held by individual article author(s).
Volume
6
Item Size
28.9M
Abstract
Freshwaters represent the most threatened environments with regard to biodiversity loss and, therefore, there is a need for national monitoring programs to effectively document species distribution and evaluate potential risks for vulnerable species. The monitoring of species for effective management practices is, however, challenged by insufficient data acquisition when using traditional methods. Here we present the application of environmental DNA (eDNA) metabarcoding of amphibians in combination with quantitative PCR (qPCR) assays for an invasive pathogenic chytrid species (Batrachochytrium dendrobatidis -Bd), a potential threat to endemic and endangered amphibian species. Statistical comparison of amphibian species detection using either traditional or eDNA-based approaches showed weak correspondence. By tracking the distribution of Bd over three years, we concluded that the risk for amphibian extinction is low since Bd was only detected at five sites where multiple amphibians were present over the sampled years. Our results show that eDNA-based detection can be used for simultaneous monitoring of amphibian diversity and the presence of amphibian pathogens at the national level in order to assess potential species extinction risks and establish effective management practices. As such our study represents suggestions for a national monitoring program based on eDNA.
Addeddate
2025-05-08 21:23:24
Bhl_virtual_titleid
210921
Bhl_virtual_volume
v.6 (2022)
Call number
10_3897_mbmg_6_85199
Call-number
10_3897_mbmg_6_85199
Foldoutcount
0
Genre
article
Identifier
nationalednabas6osma
Identifier-ark
ark:/13960/s2xn0jk9sxd
Identifier-bib
10_3897_mbmg_6_85199
Identifier-doi
10.3897/mbmg.6.85199


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